熒光的方法和應(yīng)用是熒光光譜、成像、熒光探針、標(biāo)簽和材料領(lǐng)域的原始研究文章、綜述文章和技術(shù)說(shuō)明的論壇。它的重點(diǎn)是方法和（高級(jí)）應(yīng)用。以下主題是其報(bào)道的代表。光譜學(xué)：生物分子的發(fā)光光譜，天然和合成熒光團(tuán)，熒光壽命和偏振；共振能量轉(zhuǎn)移（FRET）；相關(guān)光譜；猝滅；上轉(zhuǎn)換；等離子體相互作用；多光子激發(fā)；單分子光譜，激光誘導(dǎo)熒光光譜，高級(jí)組件和儀器、納米測(cè)量等。成像：熒光顯微鏡；多光子顯微鏡、定量熒光顯微鏡（如FLIM、FCS、FRAP、TIRF）；單分子顯微鏡；超分辨率顯微鏡（如STED、PALM、STORM、GSD、SIM）、3D成像；單平面照明顯微鏡、體內(nèi)熒光成像；先進(jìn)的成像設(shè)備和系統(tǒng)等。標(biāo)簽、探針和傳感器：蛋白質(zhì)和DNA標(biāo)簽；插層器；神經(jīng)遞質(zhì)和激素標(biāo)簽；離子探針；發(fā)光螯合物和金屬有機(jī)探針；生物結(jié)合；鑭系熒光、熒光蛋白質(zhì)；膜和脂質(zhì)探針；（分子）化學(xué)傳感器和生物傳感器、酶探針；（生物）分析（診斷，排序，信號(hào)，篩選）等。材料：包括各種點(diǎn)的納米材料；發(fā)光納米顆粒；納米管；團(tuán)簇；發(fā)光聚合物、鑭系發(fā)光材料等。該雜志不會(huì)考慮概述熒光的常規(guī)用途（例如，在檢測(cè)中）、純描述性工作或合成的文章，而不演示其用途。

Methods and Applications in Fluorescence is the forum for original research articles, review articles and technical notes in the area of fluorescence spectroscopy, imaging, fluorescent probes, labels and materials. Its focus is on both methods and (advanced) applications. The following topics are representative of its coverage.Spectroscopy: Luminescence spectroscopy of biomolecules, of natural and synthetic fluorophores, fluorescence lifetime and polarization; resonance energy transfer (FRET); correlation spectroscopy; quenching; upconversion; plasmonic interactions; multiphoton excitation; single-molecule spectroscopy, laser-induced fluorescence spectroscopy, advanced components and instrumentation, nanometry, etc.Imaging: Fluorescence microscopy; multi-photon microscopy, quantitative fluorescence microscopy (such as FLIM, FCS, FRAP, TIRF); single-molecule microscopy; super resolution microscopy (such as STED, PALM, STORM, GSD, SIM), 3D imaging; single plane illumination microscopy, in vivo fluorescence imaging; advanced devices and systems for imaging, etc.Labels, probes and sensors: Protein and DNA labels; intercalators; labels for neurotransmitters and hormones; probes for ions; luminescent chelates and metal—organic probes; bioconjugation; lanthanide fluorescence, fluorescent proteins; membrane and lipid probes; (molecular) chemical sensors and biosensors, enzyme probes; (bio)assays (diagnostic, sequencing, signalling, screening), etc.Materials: Nanomaterials including all kinds of dots; luminescent nanoparticles; nanotubes; clusters; luminescent polymers, lanthanide luminescence, etc.The journal will not consider articles outlining routine uses of fluorescence, (for example, in detection), purely descriptive work, or synthesis without a demonstration of its use.