熒光的方法和應用是熒光光譜、成像、熒光探針、標簽和材料領域的原始研究文章、綜述文章和技術說明的論壇。它的重點是方法和（高級）應用。以下主題是其報道的代表。光譜學：生物分子的發光光譜，天然和合成熒光團，熒光壽命和偏振；共振能量轉移（FRET）；相關光譜；猝滅；上轉換；等離子體相互作用；多光子激發；單分子光譜，激光誘導熒光光譜，高級組件和儀器、納米測量等。成像：熒光顯微鏡；多光子顯微鏡、定量熒光顯微鏡（如FLIM、FCS、FRAP、TIRF）；單分子顯微鏡；超分辨率顯微鏡（如STED、PALM、STORM、GSD、SIM）、3D成像；單平面照明顯微鏡、體內熒光成像；先進的成像設備和系統等。標簽、探針和傳感器：蛋白質和DNA標簽；插層器；神經遞質和激素標簽；離子探針；發光螯合物和金屬有機探針；生物結合；鑭系熒光、熒光蛋白質；膜和脂質探針；（分子）化學傳感器和生物傳感器、酶探針；（生物）分析（診斷，排序，信號，篩選）等。材料：包括各種點的納米材料；發光納米顆粒；納米管；團簇；發光聚合物、鑭系發光材料等。該雜志不會考慮概述熒光的常規用途（例如，在檢測中）、純描述性工作或合成的文章，而不演示其用途。

Methods and Applications in Fluorescence is the forum for original research articles, review articles and technical notes in the area of fluorescence spectroscopy, imaging, fluorescent probes, labels and materials. Its focus is on both methods and (advanced) applications. The following topics are representative of its coverage.Spectroscopy: Luminescence spectroscopy of biomolecules, of natural and synthetic fluorophores, fluorescence lifetime and polarization; resonance energy transfer (FRET); correlation spectroscopy; quenching; upconversion; plasmonic interactions; multiphoton excitation; single-molecule spectroscopy, laser-induced fluorescence spectroscopy, advanced components and instrumentation, nanometry, etc.Imaging: Fluorescence microscopy; multi-photon microscopy, quantitative fluorescence microscopy (such as FLIM, FCS, FRAP, TIRF); single-molecule microscopy; super resolution microscopy (such as STED, PALM, STORM, GSD, SIM), 3D imaging; single plane illumination microscopy, in vivo fluorescence imaging; advanced devices and systems for imaging, etc.Labels, probes and sensors: Protein and DNA labels; intercalators; labels for neurotransmitters and hormones; probes for ions; luminescent chelates and metal—organic probes; bioconjugation; lanthanide fluorescence, fluorescent proteins; membrane and lipid probes; (molecular) chemical sensors and biosensors, enzyme probes; (bio)assays (diagnostic, sequencing, signalling, screening), etc.Materials: Nanomaterials including all kinds of dots; luminescent nanoparticles; nanotubes; clusters; luminescent polymers, lanthanide luminescence, etc.The journal will not consider articles outlining routine uses of fluorescence, (for example, in detection), purely descriptive work, or synthesis without a demonstration of its use.